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Product Description
MDH4 | Malate dehydrogenase 4 (cytoplasmic) | AS15 3065 | Agrisera
Immunogen: Recombinant MDH4 of Zea mays, UniProt: Q08062
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: AS15 3064 | Anti-MDH2 | Malate dehydrogenase 2, mitochondrial, rabbit antibodiesantibodies to proteins involved in carbohydrate metabolism
Recommended Dilutions: 1 : 1000 (WB)
Molecular weight: 33, 5 kDa
Confirmed Reactivity: Zea mays
Predicted Reactivity: Arabidopsis thaliana, Brachypodium distachyon, Brassica oleracea, Capsella rubella, Catharanthus roseus, Erythranthe guttata, Glycine max, Gossypium hirsutum, Hordeum vulgare, Jatropha curcas, Malus domestica, Nicotiana tabacum, Oryza sativa, Prunus peersica, Ricinus communis, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera Species of your interest not listed? Contact us
Not reactive in: N/A
Additional Information: Immunoprecipitation was performed by using Dynabeads ® Protein A: briefly 100 µl suspension was washed with 200 µl TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7.6 and 165 mM NaCl with 0.1% Tween 80) using the magnetic stands for concentrating the magnetic beads. After wash the beads were preincubated with 20 µl primary antibodies in 180 µl TTBS at room temperature for 30 minutes (minimum 15 minues) . A first wash was followed afterwards with 200 µl TTBS and hence a real incubation with 200 µl plant extract (supernatant 20, 000 x g for 3 min.), 200µl of TTBS and further 50 µl YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane. This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension. Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 µl TTBS were performed prior mixing with 100 µl SDS-Sample buffer.Antibody is also binding to recombinant MHD4 (see image below) .
Background: Malate dehydrogenase 4 (cytoplasmic), (EC:1.1.1.37) display catalytic activity in conversion of (S) -malate + NAD+ = oxaloacetate + NADH.
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Carbohydrates
Research Area: Plant Biology
