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Product Description
BFP, GFP, YFP | Fluorescent Protein, clone 3A6 | AS20 4485 | Agrisera
Immunogen: Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Host: Mouse
Conjugation: N/A
Clonality: Monoclonal
Isotype: IgG1
Purity: Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Format: Lyophilized
Tested Application: ELISA (ELISA), Western blot (WB)
Related Products: AS18 4175 | Anti-GFP | Green Fluorescent Protein (monoclonal), mouse antibodies AS15 2987 | Anti-GFP (affinity purified), rabbit antibodyAS15 3000 | Anti-GFP (protein A purified), rabbit antibodyAS15 2999 | Anti-GFP (total IgG), rabbit antibodyAS15 3001 | Anti-GFP, peroxidase conjugated, rabbit antibodyAS15 3002 | GFP (purified recombinant protein) Other GFP-tag antibodies.Other tag antibodies.
Recommended Dilutions: 1 : 150-1: 3000 (ELISA), 1 : 500-1 : 5000 (WB)
Molecular weight: N/A
Confirmed Reactivity: BFP, GFP, YFP
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: N/A
Background: Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Reconstitution: Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%) . Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0, Additional sodium azide ( up to 0.05%) is recommended for long term storage., For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 µl buffer.
Storage: Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: GFP/YFP/RFP/CFP
Research Area: Tag Antibodies
