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Product Description
mCherry (monoclonal) | AS16 3967 | Agrisera
Immunogen: Recombinant full length mCherry expressed and purified from E. coli.
Host: Mouse
Conjugation: N/A
Clonality: Monoclonal
Isotype: IgG2a
Purity: IgG2a purified from tissue culture supernatant.
Format: Lyophilized
Tested Application: Immunocytochemistry (ICC) (IC), Western blot (WB)
Related Products: AS15 2987 | Anti-GFP (affinity purified), rabbit antibodiesAS15 2999 | Anti-GFP (total IgG), rabbit antibodiesAS15 3000 | Anti-GFP (protein A purified) rabbit antibodiesAS15 2998 | Anti-GFP (total IgY), chicken antibodiesAS15 3002 | GFP (purified recombinant protein) other tag antibodies
Recommended Dilutions: 1 : 250-1 : 500 (IC), 1 : 1000-1 : 2000 (WB)
Molecular weight: N/A
Confirmed Reactivity: mCherry
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector
Background: mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer) . The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals) .
Reconstitution: For reconstitution add 100 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: GFP/YFP/RFP/CFP
Research Area: Tag Antibodies
