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Product Description
Nitrotyrosine (mouse monoclonal) (clone EM-30) | AS21 4622 | Agrisera
Immunogen: NO2-Tyr-CH2-Thyroglobulin
Host: Mouse
Conjugation: N/A
Clonality: Monoclonal
Isotype: IgG1
Purity: Immunoglobulin purified on Protein A.
Format: Liquid at 1 mg/ml.
Tested Application: Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)
Related Products: N/A
Recommended Dilutions: (IHC) paraffin: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia. (WB) : positive control: NO2-BSA, NO2-HSA.
Molecular weight: N/A
Confirmed Reactivity: Nitrotyrosine
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: N/A
Background: Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.
Reconstitution: N/A
Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Oxidative stress
Research Area: Proteomics
