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Product Description
AGO1 | Argonaute 1 (40 µg, HRP-conjugated) | AS09 527-HRP | Agrisera
Immunogen: KLH-conjugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR: At1g48410.
Host: Rabbit
Conjugation: KLH
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
Format: Liquid
Tested Application: Western blot (WB)
Related Products: AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibodiesAS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated), rabbit antibodiesAS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated), rabbit antibodiesAS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibodiesAS09 527P | AGO1 | argonaute 1 | Blocking peptideAS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibodiesAS09 617 | Anti-AGO4 argonaute 4, rabbit antibodiesAS10 671 | Anti-AGO5 | argonaute 5, rabbit antibodiesAS10 672 | Anti-AGO6 | argonaute 6, rabbit antibodiesAS10 673 | Anti-AGO9 | argonaute 9, rabbit antibodiesAS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibodiescollection of antibodies to micro RNAPlant protein extraction bufferSecondary antibodies
Recommended Dilutions: 1 : 1000 (WB)
Molecular weight: 116, 4 | 130 kDa
Confirmed Reactivity: Arabidopsis thaliana, Nicotiana benthamiana
Predicted Reactivity: Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Not reactive in: Chlamydomonas reinhardtii
Additional Information: AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC) . This protein complex is responsible for the gene silencing (RNAi) .This antibody is directly conjugated to Horseradish Peroxidase (HRP) .
Reconstitution: N/A
Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
TAIR Nnumbre: AT1G48410
Category: microRNA
Research Area: RNAi/siRNA/miRNA
