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Product Description
Cas9 | CRISPR-associated endonuclease 9 (polyclonal) | AS16 3690 | Agrisera
Immunogen: KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6 (peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Tested Application: Immunolocalization (IL), Western blot (WB)
Related Products: AS16 3841 | Anti/Cpf1 | CRISPR from Prevotella and Francisella 1, rabbit antibodiesCollection of antibodies to tag proteinsPlant and algal protein extraction buffer Secondary antibodies
Recommended Dilutions: 1 : 100 (IL), 1 : 1000 (WB)
Molecular weight: Depends upon a MW of a protein which is a fusion partner,
Confirmed Reactivity: Staphylococcus aureus
Predicted Reactivity: Staphylococcus pyogenes UniProt: Q99ZW2
Not reactive in: N/A
Additional Information: N/A
Background: Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) . Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: CRISPR
Research Area: Gene Editing/CRISPR
