Product Description
GDC-H | H protein of glycine decarboxylase complex (GDC) | AS05 074 | Agrisera
Immunogen: purified GDC-H protein from Spinacia oleracea
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Total IgG. Protein G purified in PBS pH 7.4.
Format: Lyophilized
Tested Application: Tissue printing (TP), Western blot (WB)
Related Products: AS06 203A | Anti- Idh | isocytrate dehydrogenase rabbit antibodies, marker of mitochondrial matrixAS07 212 | Anti-VDAC1 marker, rabbit antibodies for mitochondrial outer membranePlant protein extraction buffer Secondary antibodies
Recommended Dilutions: 1 : 5 000 (TP), (WB)
Molecular weight: 16 kDa
Confirmed Reactivity: Arabidopsis thaliana, Petunia hybrida cv. Mitchell, Portulaca grandiflora, Spinacia oleracea, Triticum aestivum, Vicia faba
Predicted Reactivity: higher plants
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: This antibody can be used on total cell extract of Arabidopsis thaliana.
Background: The Glycine decarboxylase complex (GDC) is abundant in mitochondria matrix of C3 leaves and functions in photorespiratory carbon recovery. GDC enzyme can account for up to 50% of matrix protein, and is responsible for the most prominent metabolic activity in the mitochondria of illuminated leaves, photorespiration. GDC is a multienzyme complex composed of four component enzymes, the P-, H-, T-, and L-proteins and is responsible for the conversion of glycine produced in the peroxisome to serine in the mitochondria during photorespiratory cycle. The H-protein plays a key role as a mobile substrate that commutes between the other subunits, allowing its lipoic acid "arm" to visit the active sites of the other three components.
Reconstitution: For reconstitution add 200 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT2G35370
Category: Mitochondria | Respiration
Research Area: Plant Biology