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Product Description
GFP | Green Fluorescence Protein | AS20 4443 | Agrisera
Immunogen: Recombinant GFP protein derived from Aequorea victoria, UniProt: P42212
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Tested Application: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)
Related Products: Collection of antibodies to tags
Recommended Dilutions: 1: 5000 - 1 : 10 000 (WB)
Molecular weight: N/A
Confirmed Reactivity: Recombinant GFP overexpressed in E.coli, from: Arabidopsis thaliana, Chlamydomonas reinhardii, Nicotiana tabacum
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: N/A
Background: GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: TAIR
Category: GFP/YFP/RFP/CFP
Research Area: Tag Antibodies
