Product Description
H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade) | AS16 3195 | Agrisera
Immunogen: KLH-conjugated synthetic peptide
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum.
Format: Liquid
Tested Application: Chromatin immunoprecipitation (ChIP/ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
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Recommended Dilutions: 0, 5-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 500 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
Molecular weight: 15 kDa
Confirmed Reactivity: Human
Predicted Reactivity: Arabidopsis thaliana, Mouse, Rat
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Antibody solution contains PBS with 0, 05% sodium azide and 0, 05% ProClin 300
Background: Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.
Reconstitution: N/A
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Epigenetics/DNA methylation
Research Area: Epigenetics