Product Description
H3K27me3S28p | Histone H3 (trimethylated Lys27, p Ser28) | AS16 3184 | Agrisera
Immunogen: KLH-conjugated synthetic peptide
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum.
Format: Liquid
Tested Application: Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)
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Recommended Dilutions: 2-5 µg/million cells (ChIP), 1 : 500-1 : 1000 (Dot), 1 : 200 (IF), 1 : 50 (IHC), 1 : 500 (WB)
Molecular weight: 15 kDa
Confirmed Reactivity: Human
Predicted Reactivity: Caenorhabditis elegans, Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: N/A
Background: Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, trimethylation of K27 is associated with gene silencing, whereas pS28 is associated with mitotis and immediate early genes.
Reconstitution: N/A
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Epigenetics/DNA methylation
Research Area: Epigenetics