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Product Description
H3T3pK4ac | Histone H3 (ac Lys4, p Thr3) | AS16 3170 | Agrisera
Immunogen: KLH-conjugated synthetic acetylated/phosphorylated peptide surrounding Lysine 4 and Threonine 3 of human Histone H3
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum.
Format: Liquid
Tested Application: Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Related Products: AS16 3168 | Anti-H3T3pK4me1 | Histone H3 (p Thr3, monomethyl Lys4), polyclonal antibodies AS16 3197 | Anti-anti-H3core, monoclonal antibodiesAS15 2855 | Anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | Anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710collection of antibodies to epigenetics
Recommended Dilutions: 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 100 (IF), 1 : 50 (IHC), 1 : 500 (WB)
Molecular weight: 15 kDa
Confirmed Reactivity: Caenorhabditis elegans, Human
Predicted Reactivity: Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: N/A
Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin) . Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Phosphorylation of threonine 3 (H3T3p) is a known mitotic marker and modified by the Haspin/Thr3 enzyme, while acetylation of lysine 4 (H3K4ac) on histone 3 is associated with transcriptional activation by Esa1. Alternative names: H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3pT3/K4ac .
Reconstitution: N/A
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Epigenetics/DNA methylation
Research Area: Epigenetics
