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Product Description
mNeonGreen | Fluorescent Protein | AS21 4525 | Agrisera
Immunogen: KLH-conjugated peptide derived from synthetic peptide, UniProt: A0A1S4NYF2 from common lancelet Branchiostoma lanceolatum
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum, in PBS pH 7.4.
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: Collection of epitope tag antibodies to GFP/YFP/RFP/CFP
Recommended Dilutions: 1 : 1000 - 1: 5000 (WB)
Molecular weight: Depends upon used fusion partner
Confirmed Reactivity: mNG tag in plant (Arabidopsis thaliana) and algal vectors (Chlamydomonas reinhardtii)
Predicted Reactivity: Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
Additional Information: This antibody is detecting protein sequence of mNG tag in plant and algal vectors.This antibody is also reacting to some degree with YFP and mCherry.
Background: mNeonGreen (Fluorescent Protein) is a new bright monomertic yellow-green fluorescent, with low conservation level to GFP. It has an excitation maximum at 506 nm and an emission maximum at 517 nm and therefore is compatible with the most GFP filter sets. mNeonGreen is 3x brighter compare to GFP, more stable and does not bleach so fast as GFP, which makes it very suitable for confocal and super resolution microscopy, for fusion proteins with low expression levels. It can be used in bicistronic vectors, which will allow simultaneous expression of two proteins separately, from the same RNA transcript. mNeonGreen has MW of 26.6 kDa.
Reconstitution: For reconstitution add 50 µl, of sterile water.
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: TAIR
Category: GFP/YFP/RFP/CFP
Research Area: Tag Antibodies
