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Product Description
UmuD | Protein UmuD | AS21 4546 | Agrisera
Immunogen: Highly purified, full length, recombinant UmuD protein from E.coli, UniProt: P0AG11
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum. Contains 0.05 % sodium azide.
Format: Liquid
Tested Application: Western blot (WB)
Related Products: Collection of antibodies to bacterial proteins
Recommended Dilutions: 1 : 3000 (WB)
Molecular weight: 17 kDa (intact) 14 kDa (processed)
Confirmed Reactivity: Escherichia coli
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: N/A
Background: The products of umuD, umuC, and recA genes (SOS genes) are required for mutagenesis induced by radiation or chemical agents. Transcription of these SOS genes is repressed by a repressor, LexA protein in uninduced cells. Exposure of cells to DNA-damaging agents activates RecA protein to promote proteolytic cleavage of LexA protein. Inactivation of LexA protein by the cleavage consequently derepresses the SOS genes, umuD, C and recA . UmuD protein is then auto-cleaved, which is promoted by RecA protein ssDNA in a ATP-dependent manner. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as an error-prone translesion DNA polymerase.
Reconstitution: N/A
Storage: Store at -20°C and make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Bacteria
Research Area: Bacteria
