Synthetic peptide from the N-terminal of Human RAC-alpha serine/threonine-protein kinase (aa. 1-100)
Immunogen Species:
Human
Applications:
WB, IHC
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human, Mouse, Rat
Antibody Dilution:
WB (1:1000); IHC (1:50); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration:
1 mg/ml
Specificity:
Detects 55 kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:1000 dilution of SPC-789 was sufficient for detection of AKT1 in 15 µg of rat Liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization:
Cytoplasm, Nucleus, Cell Membrane
Tissue Specificity:
Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer
Certificate of AnalysisA 1:1000 dilution of SPC-789 was sufficient for detection of AKT1 in 15 µg of rat Liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Tissue SpecificityExpressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer