Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, PKB / AKT, Cancer, Apoptosis, Cell Signaling, Metabolism, Drug Metabolism
Alternative Name:
AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody
Category:
Antibodies
Product Type:
Polyclonal
Immunogen:
Synthetic peptide of human Akt1 (AA133-147)
Immunogen Species:
Human
Applications:
WB, AM
Isotype:
NA
Species Reactivity:
Human, Mouse
Antibody Dilution:
WB (1:250); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Concentration:
NA
Specificity:
Detects 55.716 kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:250 dilution of SPC-1100 was sufficient for detection of AKT1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization:
Cytoplasm, Nucleus, Cell Membrane
Accession Number:
NP_005154
Gene ID:
207
Swiss-Prot:
P31749
Tissue Specificity:
Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer
Certificate of AnalysisA 1:250 dilution of SPC-1100 was sufficient for detection of AKT1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Tissue SpecificityExpressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer