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Product Description
Rabbit Anti-Human AKT1 (pTyr315) Polyclonal
Product Videos
Custom Field
Size 100 µl
Target AKT1 (pTyr315)
Conjugate FITC
Research Area Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | PKB / AKT | Cancer | Apoptosis | Cell Signaling | Metabolism | Metabolism processes
Alternative Name AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody
Category Antibodies
Product Type Polyclonal Antibody
Immunogen A phospho-specific peptide corresponding to residues surrounding Tyr315 of human Akt1 (AA312-318)
Immunogen Species Human
Applications WB, AM
Host Rabbit
Isotype N/A
Species Reactivity Human
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Concentration N/A
Specificity Detects 55.686 kDa.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:250 dilution of SPC-911 was sufficient for detection of AKT1 (pTyr315) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm, Nucleus, Cell Membrane
Tissue Specificity Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer
Accession Number NP_005154
Gene ID 207
Swiss-Prot P31749
Field of Use For in vitro research use only.
