Synthetic peptide of Human Alpha Synuclein pSer129 (40-140 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Immunogen Species:
Human
Applications:
WB, ELISA, ICC/IF, IHC
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human, Mouse, Rat
Antibody Dilution:
WB (1:1000); ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration:
0.5mg/mL
Specificity:
Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:1000 dilution of SPC-742 was sufficient for detection of Alpha Synuclein pSer129 in 15 µg of human brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
SpecificityBinds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:1000 dilution of SPC-742 was sufficient for detection of Alpha Synuclein pSer129 in 15 µg of human brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Tissue SpecificityHighly expressed in brain (presynaptic nerve terminals) but is also expressed in low concentrations in all tissues examined except in liver.