Neuroscience, Cell Markers, Neuron Markers, Cytoplasmic Markers, Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Domain Families, Cancer, Tumor Biomarkers, Oncoproteins, Cell Signaling, Epigenetics and Nuclear Signaling, Chromatin M
Alternative Name(s):
c-fos Antibody, Activator protein 1 antibody, AP 1 antibody, C FOS antibody, Cellular oncogene c fos antibody, Cellular oncogene fos antibody, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody, FBJ murine osteosarcoma vir
Product Type:
Monoclonal Antibody
Clone Number:
5B11
Immunogen:
Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380)
Immunogen Species:
Human
Applications:
WB
Host:
Mouse
Isotype:
IgG2b
Species Reactivity:
Human
Antibody Dilution:
WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration:
1 mg/ml
Specificity:
Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:1000 dilution of SMC-545 was sufficient for detection of c-fos in 10 µg of serum starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization:
Cytosol | Endoplasmic reticulum | Nucleus
Accession Number:
NP_005243.1
Gene ID:
2353
Swiss-Prot:
P01100
Field of Use:
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
SpecificityDetects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:1000 dilution of SMC-545 was sufficient for detection of c-fos in 10 µg of serum starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.