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Product Description
Rabbit Anti-Human Collagen I Polyclonal
Product Videos
Size
Size 100 µl
Target Collagen I
Conjugate Unconjugated
Research Area Cell Signaling | Extracellular Matrix (ECM) | Collagen | Stem Cells | Mesenchymal Stem Cells | Osteogenesis
Alternative Name Alpha 1 type I collagen Antibody, Alpha 2 type I collagen Antibody, CO1A1_HUMAN Antibody, collagen alpha 1 chain type I Antibody, EDSC Antibody, Type I procollagen antibody
Category Antibodies
Product Type Polyclonal Antibody
Immunogen Synthesized peptide derived from N-terminal of human Collagen I.
Immunogen Species Human
Applications IHC, ICC/IF, ELISA
Host Rabbit
Isotype IgG
Species Reactivity Human, Mouse, Rat
Antibody Dilution IHC (1:100), ICC/IF (1:500), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Concentration N/A
Specificity Detects ~139kDa, endogenous levels of total Collagen I protein. Collage type I is a triple helix consisting of one alpha-2 and 2 alpha-1 chains, so dimeric (~270kDa) and trimeric (~400kDa) forms may be expected.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:100 dilution of SPC-1273 was sufficient for detection of Collagen I in NIH/3T3 cells using Goat Anti-Rabbit IgG as the secondary antibody.
Cellular Localization Extracellular matrix
Tissue Specificity Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Accession Number NP_000080.2
Gene ID 1278
Swiss-Prot P08123
Field of Use For in vitro research use only.
