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Product Description
Rabbit Anti-Human Collagen II Polyclonal
Product Videos
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Size 100 µl
Target Collagen II
Conjugate Biotin
Research Area Cell Signaling | Extracellular Matrix (ECM) | Collagen | Stem Cells | Mesenchymal Stem Cells | Osteogenesis | Cell Signaling | Extracellular Matrix (ECM) | Bone
Alternative Name Alpha 1 type II collagen Antibody, AOM Antibody, Cartilage collagen Antibody, Chondrocalcin Antibody, COL11A3 Antibody, Collagen II alpha 1 polypeptide Antibody, SEDC Antibody
Category Antibodies
Product Type Polyclonal Antibody
Immunogen Synthesized peptide derived from human Collagen II.
Immunogen Species Human
Applications WB, IHC, ICC/IF, ELISA
Host Rabbit
Isotype IgG
Species Reactivity Human, Mouse, Rat
Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Concentration N/A
Specificity Detects endogenous levels of total Collagen II protein.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-1274 was sufficient for detection of Collagen II in 10 µg of COLO205 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cellular Localization Extracellular matrix
Tissue Specificity Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.
Accession Number NP_149162.2
Gene ID 1280
Swiss-Prot P02458
Field of Use For in vitro research use only.
