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Product Description
Rabbit Anti-Human Cyclin E1 (pThr395) Polyclonal
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Size 100 µl
Target Cyclin E1 (pThr395)
Conjugate Unconjugated
Research Area Cancer | Cell Cycle | Cyclins
Alternative Name Cyclin Es Antibody, cyclin E variant ex5del Antibody, Cyclin E1 Antibody, Cyclin Et Antibody, CCNE1 Antibody, CCNE Antibody, G1/S-specific cyclin-E1 Antibody, cyclin E variant ex7del Antibody, CCNE1_HUMAN Antibody, G1/S specific cyclin E Antibody, Cy
Category Antibodies
Product Type Polyclonal Antibody
Immunogen Synthetic phospho-peptide from human Cyclin E1 around the phosphorylation site of threonine 395 (pThr395).
Immunogen Species Human
Applications WB, IHC, ELISA
Host Rabbit
Isotype IgG
Species Reactivity Human
Antibody Dilution WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
Concentration N/A
Specificity Detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 395 (pThr395).
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:500 dilution of SPC-1367 was sufficient for detection of Cyclin E1 (pThr395) in 10 µg of human cervical cancer cell line (hela) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
Cellular Localization Nucleus, Cytosol, Nucleoplasm, Cyclin E1-Cdk2 Complex
Tissue Specificity Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Accession Number NP_001229.1
Gene ID 898
Swiss-Prot P24864
Field of Use For in vitro research use only.
