Product Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. While GAPDH has long been recognized as playing an integral role in glycolysis, additional functions of GAPDH include acting as a uricil DNA glycosylase, activating transcription, binding RNA and involvement in nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH is upregulated in liver, lung and prostate cancers. GAPDH translocates to the nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated in human neuro-degenerative disorders including the b-Amyloid precursor, Huntingtin and other triplet repeat neuronal disorder proteins.
Biovision | 3777R | GAPDH Antibody DataSheet
Antibody Target: GAPDH
Target Alternative Name: GAPDH, G3PD, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
Tag Line:
Category: GADPH
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human, mouse, rat
Immunogen Sequence: Synthetic peptide surrounding amino acid 317of rat GADPH
Accession #: XP_214281.3
Gene ID: 290604
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.5 mg/ml
Formulation: 30 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Purification: Affinity purified
Application: Western blot
Positive Control:
Application And Usages: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes over 100 kDa band corresponding to NIF-1 in samples from human origin. Reactivity to other species has not been tested.
Country of Animal Origin: China
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified: Not Verified
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified