Detects endogenous levels of GSK3a only when phosphorylated at serine 21. (pSer21).
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:500 dilution of SPC-1385 was sufficient for detection of GSK3a (pSer21) in 10 µg of human ovary cancer cell line (a2780) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
ImmunogenSynthetic phospho-peptide from human GSK3a around the phosphorylation site of serine 21. (pSer21).
Immunogen SpeciesHuman
ApplicationsWB, IHC, ELISA, IP
HostRabbit
IsotypeIgG
Species ReactivityHuman, Mouse, Rat
Antibody DilutionWB (1:250-1:500); IHC (1:25-1:50); ELISA (1:10000); IP (Various dilutions); optimal dilutions for assays should be determined by the user.
SpecificityDetects endogenous levels of GSK3a only when phosphorylated at serine 21. (pSer21).
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:500 dilution of SPC-1385 was sufficient for detection of GSK3a (pSer21) in 10 µg of human ovary cancer cell line (a2780) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.