WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~78kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization:
Endoplasmic Reticulum
Accession Number:
N/A
Gene ID:
N/A
Swiss-Prot:
N/A
Field of Use:
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Research AreaCancer, Heat Shock, Cell Signaling, Protein Trafficking
Alternative Name(s)H-D-E-L (his-asp-glu-leu) Antibody, endoplasmic reticulum Antibody, luminal ER protein retention Antibody, KDELR1 Antibody, Endoplasmic reticulum retention signal Antibody
Product TypeMonoclonal Antibody
Clone Number2E7
ImmunogenRaised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
Certificate of Analysis1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular LocalizationEndoplasmic Reticulum
Accession NumberN/A
Gene IDN/A
Swiss-ProtN/A
Field of UseNot for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.