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Product Description
Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal
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Size 100 µl
Target Histone H2A.X (pSer139)
Conjugate HRP
Research Area Cell Signaling | Epigenetics and Nuclear Signaling | Histones | DNA/RNA | DNA Damage and Repair | DNA Damage Recognition
Alternative Name H2A histone family member X Antibody, H2A.FX Antibody, H2A.X Antibody, H2AFX Antibody, H2AX histone Antibody, Histone 2A Antibody, Histone 2AX Antibody
Category Antibodies
Product Type Polyclonal Antibody
Immunogen Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
Immunogen Species Human
Applications WB, ICC/IF
Host Rabbit
Isotype IgG
Species Reactivity Human, Mouse, Rat
Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Concentration N/A
Specificity Detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-1251 was sufficient for detection of Histone H2A.X in 10 µg of 293T cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus, Chromosome
Tissue Specificity N/A
Accession Number NP_002096.1
Gene ID 3014
Swiss-Prot P16104
Field of Use For in vitro research use only.
