Product Description
Rat Anti-Mouse HSF1 Monoclonal IgG1
Size
Size 100 µg
Other name HSF1 Antibody
Host Rat
Target HSF1
Conjugate Unconjugated
Research Area Cancer, Heat Shock, Cell Signaling, Epigenetics and Nuclear Signaling, Cardiovascular System, Heart
Alternative Name HSTF1 Antibody, Heat shock factor protein 1 Antibody, Heat shock transcription factor 1 Antibody, HSF 1 Antibody
Product Type Monoclonal Antibody
Clone Number 10H8
Immunogen Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
Immunogen Species Mouse
Applications WB, IHC, ICC/IF, IP, ELISA, GS
Isotype IgG1
Species Reactivity Human, Mouse, Rat, Bovine, Guinea Pig (Cavia porcellus), Hamster, Monkey, Rabbit
Antibody Dilution WB (1:1000), IHC (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Specificity Detects ~85kDa (unstressed cell lysates), and~95kDa (heat shocked cell lysates).
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-118 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody
Cellular Localization Cytoplasm, Nucleus
Tissue Specificity N/A
Accession Number NP_032322.1
Gene ID 15499
Swiss-Prot P38532