Product Description
Rat Anti-Mouse HSF1 Monoclonal IgG1
Size
Size 100 µg
Other Name HSF1 Antibody
Target HSF1
Conjugate Unconjugated
Research Area Cancer, Heat Shock, Cell Signaling, Epigenetics and Nuclear Signaling, cardiovascular System, Heart
Alternative Name(s) HSTF1 Antibody, Heat shock factor protein 1 Antibody, Heat shock transcription factor 1 Antibody, HSF 1 Antibody
Product Type Monoclonal Antibody
Clone Number 4B4
Immunogen Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439
Immunogen Species Mouse
Applications WB, IHC, ICC/IF, IP, ELISA, GS
Host Rat
Isotype IgG1
Species Reactivity Human, Mouse, Rat, Bovine, Guinea Pig (Cavia porcellus), Hamster, Monkey, Rabbit
Antibody Dilution WB (1:1000), IHC (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Concentration 1 mg/ml
Specificity Detects ~85kDa (unstressed cell lysates) and ~95kDa (heat shocked cell lysates).
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-477 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus
Accession Number NP_032322.1
Gene ID 15499
Swiss-Prot P38532
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.