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Product Description
Mouse Anti-Human HSP27 Monoclonal IgG2b Kappa
Product Videos
Custom Field
Size 200 µg
Other Name HSP27 Antibody: APC/Cy7
Target HSP27
Conjugate APC/Cy7
Research Area Cell Signaling, Protein Trafficking, Chaperone Proteins, Cell Signaling, Cytoskeleton, Microfilaments, Actin, Actin Assembly, Cell Signaling, Cytoskeleton, Microtubules, Cancer, Tumor Biomarkers, Cardiovascular System, Atherosclerosis, Cardiovascular
Alternative Name(s) 28kDa heat shock protein Antibody, CMT2F Antibody, HSP25 Antibody, HSP27 Antibody, HSP28 Antibody, HSPB1 Antibody, SRP27 Antibody
Product Type Monoclonal Antibody
Clone Number 5D12-A12
Immunogen Full length human HSP27
Immunogen Species Human
Applications WB, IHC, ICC/IF, IP, ELISA
Host Mouse
Isotype IgG2b Kappa
Species Reactivity Human
Antibody Dilution WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Specificity Detects ~27kDa. Has no cross-reactivity to Alpha B crystallin. Very limited cross-reactivity to other species.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis 0.5 µg/ml of SMC-161 was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus | Cytoskeleton | Spindle
Accession Number NP_001531.1
Gene ID 3315
Swiss-Prot P04792
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
