Product Description
Mouse Anti-Human HSP25/HSP27 Monoclonal IgG1 Kappa
Size
Size 100 µg
Other name HSP25/HSP27 Antibody
Host Mouse
Target HSP27
Conjugate Unconjugated
Research Area Cell Signaling, Protein Trafficking, Chaperone Proteins, Cell Signaling, Cytoskeleton, Microfilaments, Actin, Actin Assembly, Cell Signaling, Cytoskeleton, Microtubules, Cancer, Tumor Biomarkers, Cardiovascular System, Atherosclerosis, Cardiovascular
Alternative Name HSPB2 Antibody, Heat shock protein beta-2 Antibody, HSPB2 Antibody, DMPK-binding protein Antibody, MKBP Antibody, HSP27 Antibody, HSP25 Antibody
Product Type Monoclonal Antibody
Clone Number 8A7
Immunogen HSP27 peptide
Immunogen Species Human
Applications WB, IHC, ICC/IF, IP, FCM, FACS
Isotype IgG1 Kappa
Species Reactivity Human, Mouse, Rat, Bovine, Dog, Guinea Pig (Cavia porcellus), Hamster, Sheep
Antibody Dilution WB (1:5000), ICC/IF (1:200), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Specificity Detects ~25kDa or ~27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization Cytoplasm, Nucleus, Cytoskeleton, Spindle
Tissue Specificity N/A
Accession Number NP_001532.1
Gene ID 3315
Swiss-Prot P04792