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Product Description
Rabbit Anti-Human HSP27 (pSer15) Polyclonal
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Size 100 µl
Target HSP27 (pSer15)
Conjugate APC/Cy7
Research Area Cell Signaling | Protein Trafficking | Chaperone Proteins | Cell Signaling | Cytoskeleton | Microfilaments | Actin | Actin Assembly | Cell Signaling | Cytoskeleton | Microtubules | Cancer | Tumor Biomarkers | Cardiovascular System | Atherosclerosis |
Alternative Name 28kDa heat shock protein Antibody, CMT2F Antibody, HSP25 Antibody, HSP27 Antibody, HSP28 Antibody, HSPB1 Antibody, SRP27 Antibody
Category Antibodies
Product Type Polyclonal Antibody
Immunogen Synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G-P-SP-W-D).
Immunogen Species Human
Applications WB, IHC, ICC/IF
Host Rabbit
Isotype IgG
Species Reactivity Human, Mouse, Rat
Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Concentration N/A
Specificity Detects endogenous levels of HSP27only when phosphorylated at serine 15.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-1263 was sufficient for detection of Hsp27 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm, Nucleus
Tissue Specificity Ubiquituous.
Accession Number NP_001531.1
Gene ID 3315
Swiss-Prot P04792
Field of Use For in vitro research use only.
