WB (1:4000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS, 50% glycerol, 0.1mM PMSF
Concentration:
1 mg/ml
Specificity:
Detects ~60kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
0.25 µg/ml of SMC-111 was sufficient for detection of HSP60 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.
Cellular Localization:
Mitochondrion | Mitochondrion Matrix
Accession Number:
NP_002147.2
Gene ID:
3329
Swiss-Prot:
P10809
Field of Use:
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Species ReactivityHuman, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit, Plant, Spinach, Bacteria, E. coli (Escherichia coli), H. pylori (Helicobacter pylori), Bacteria (Salmonella Typhimurium), Nematode (Trichinella spira
Antibody DilutionWB (1:4000), IHC (1:100); optimal dilutions for assays should be determined by the user.
PurificationProtein G Purified
Storage BufferPBS, 50% glycerol, 0.1mM PMSF
Concentration1 mg/ml
SpecificityDetects ~60kDa.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis0.25 µg/ml of SMC-111 was sufficient for detection of HSP60 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.