Synthetic peptide from the N-terminal of Human HSP70 Acetyl Lys77 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Immunogen Species:
Human
Applications:
WB, ELISA, ICC/IF, IHC
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human, Mouse, Rat
Antibody Dilution:
WB (1:1000); ELISA (1:1000); ICC/IF (1:60); IHC (1:50); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration:
0.5mg/mL
Specificity:
Binds to acetylated lysine 77 on HSP70. Detects 70 kDa band.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:1000 dilution of SPC-743 was sufficient for detection of HSP70 Acetyl Lys77 in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
SpecificityBinds to acetylated lysine 77 on HSP70. Detects 70 kDa band.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:1000 dilution of SPC-743 was sufficient for detection of HSP70 Acetyl Lys77 in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.