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Product Description
Mouse Anti-Human Cell Membrane HSP70 Monoclonal IgG1
Product Videos
Size
Size 100 µg
Other Name HSP70 Antibody: Biotin
Target HSP70
Conjugate Biotin
Research Area Cell Signaling, Protein Trafficking, Chaperone Proteins, Cancer, Tumor Biomarkers, Heat Shock
Alternative Name(s) HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
Product Type Monoclonal Antibody
Clone Number 1H11
Immunogen Human native HSP70 protein
Immunogen Species Human
Applications WB, ICC/IF, FCM, FACS
Host Mouse
Isotype IgG1
Species Reactivity Human, Mouse, Rat, Bovine, Pig
Antibody Dilution WB (1:1000), ICC/IF (1:100), FACS (1:250); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration 0.5 mg/ml
Specificity Conformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a ~70 kDa
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:250 dilution of SMC-249 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.
Cellular Localization Cell membrane
Accession Number NP_005336.3
Gene ID 3303
Swiss-Prot P0DMV8/P0DMV9
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
