Human, Mouse, Rat, Amphibians, Chicken, Fish, Yeast, Yeast (Saccharomyces cerevisiae), Fruit Fly (Drosophila melanogaster)
Antibody Dilution:
WB (1:1000), ICC/IF (1:500), IP (1µg); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~70kDa. May detect HSP70, HSC70, Grp78 and HSP72.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml of SMC-162 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization:
Cytoplasm
Accession Number:
NP_005336.3
Gene ID:
3303
Swiss-Prot:
P0DMV8/P0DMV9
Field of Use:
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
SpecificityDetects ~70kDa. May detect HSP70, HSC70, Grp78 and HSP72.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis1 µg/ml of SMC-162 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular LocalizationCytoplasm
Accession NumberNP_005336.3
Gene ID3303
Swiss-ProtP0DMV8/P0DMV9
Field of UseNot for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.