WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects 90kDa. Will detect both alpha (inducible) and beta (constitutively-expressed) forms.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml was sufficient for detection of HSP90αβ in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
SpecificityDetects 90kDa. Will detect both alpha (inducible) and beta (constitutively-expressed) forms.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis1 µg/ml was sufficient for detection of HSP90αβ in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.