A phospho-specific peptide corresponding to residues surrounding Tyr156 and Thr157 of human ICK (AA152-158)
Immunogen Species:
Human
Applications:
WB, AM
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human
Antibody Dilution:
WB (1:250); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Concentration:
N/A
Specificity:
Detects 71.427 kDa.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:250 dilution of SPC-996 was sufficient for detection of ICK (pTyr156 + pThr157) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization:
Nucleus, Cytoplasm, Cytosol, Cytoskeleton
Tissue Specificity:
Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines
Certificate of AnalysisA 1:250 dilution of SPC-996 was sufficient for detection of ICK (pTyr156 + pThr157) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Tissue SpecificityExpressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon, with highest levels in placenta and testis. Not detected in spleen. Also expressed in many cancer cell lines