Synthetic peptide of Human NFATC1 (110-210 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Immunogen Species:
Human
Applications:
WB, IHC
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human, Rat
Antibody Dilution:
WB (1:1000); IHC (1:50); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration:
1 mg/ml
Specificity:
Detects 90 kDa, band at ~60 kDa is another isoform.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:1000 dilution of SPC-727 was sufficient for detection of NFATC1 in 15 µg of rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization:
Cytoplasm, Nucleus
Tissue Specificity:
Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen).
SpecificityDetects 90 kDa, band at ~60 kDa is another isoform.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:1000 dilution of SPC-727 was sufficient for detection of NFATC1 in 15 µg of rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular LocalizationCytoplasm, Nucleus
Tissue SpecificityExpressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen).