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Product Description
LIMK1 is a serine-threonine protein kinase that contains 2 zinc finger motifs, known as LIM motifs at their N-terminus and a kinase sequence at their C terminus. The protein is expressed ubiquitously in different regions of the adult brain. It has reduced expression in the heart and skeletal muscles. LIMK1 prevents the cleavage of actin filament and stabilizes the actin cytoskeleton by phosphorylating and inactivating actin binding factors such as cofilin-1 (CFL1), cofilin-2 (CFL2), and destrin (DSTN). Additionally, it acts downstream of Rho family GTPase signal transduction pathways. Kinases such as ROCK1, PAK1, and PAK4 phosphorylate LIMK1 on the threonine residue in the activation loop and increase the activity of LIMK1. LIMK1 deletion is associated with the impairment of visuospatial cognition and long-term memory of Williams syndrome. Overexpression of LIMK1 is observed in multiple cancers such as breast, colorectal, and prostate cancer.
Biovision | A2228 | Anti-LIMK1 Antibody DataSheet
Antibody Target: LIMK1
Target Alternative Name: LIMK, LIM domain kinase 1, LIMK-1, EC 2.7.11.1, LIM Motif-Containing Protein Kinase, LIMK1
Tag Line: Affinity purified rabbit polyclonal antibody to detect LIMK1 expression in human, mouse and rat samples
Category: Primary antibody
Host: Rabbit
Isotype: IgG
Species Reactivities: Rat,Mouse,Human
Immunogen Sequence: KLH-conjugated synthetic peptide targeting a sequence within the center region of human LIMK1
Accession #: P53667
Gene ID: 3984
Appearance: Colorless liquid
Form: Liquid
Concentration:
Formulation: In 0.42% Potassium phosphate; 0.87% NaCl; pH 7.3; 30% glycerol; and 0.01% sodium azide
Purification: Affinity purified
Application: Immunofluorescence (IF),Western Blotting
Positive Control:
Application And Usages: WB 1:500 - 1:1000, IF 1:100 - 1:500
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: TRUE
Immunocytochemistry Verified: FALSE
Immunofluorescence Verified: TRUE
Immunoprecipitation Verified: FALSE
FACS Verified: FALSE
ELISA Verified: FALSE
ChIP Verified: FALSE
Dot Blot Verified: FALSE
Flow Cytometry Verified: FALSE
