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Product Description
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indispensible for cell proliferation and survival. Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met. In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR. Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination. It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation.
Biovision | 6658 | USP8 Antibody DataSheet
Antibody Target: USP8
Target Alternative Name: Deubiquitinating enzyme 8, hUBPy, HumORF8, KIAA0055, mgC129718, Ubiquitin carboxyl-terminal hydrolase 8, Ubiquitin-specific processing protease 8, Ubiquitin thioesterase 8, UBP, Ubiquitin Specific Protease 8.
Tag Line: Polyclonal Antibody to detect USP8 in human samples
Category: USP8
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human
Immunogen Sequence: Synthetic peptide corresponding to an internal sequence of human USP 8 (BV-L43)
Accession #: P40818
Gene ID: 9101
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.5 mg/ml
Formulation: In phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
Purification: Affinity Purified Antibody
Application: Western blot
Positive Control: Western blot: Jurkat Cell lysate
Application And Usages: Western blot: 1-4 µg/ml.
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified:
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified
