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Product Description
Rabbit Anti-Human PUMA (NT) Polyclonal
Product Videos
Size
Size 100 µg
Target PUMA
Conjugate ATTO 594
Research Area Cancer | Apoptosis | Intracellular | Bcl2 Family | Cancer | Invasion/microenvironment | Cell Signaling | Metabolism | Mitochondrial Metabolism
Alternative Name BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
Category Antibodies
Product Type Polyclonal Antibody
Immunogen N-terminal amino acids of human PUMA
Immunogen Species Human
Applications WB, IHC
Host Rabbit
Isotype N/A
Species Reactivity Human, Rat, Rainbow Trout (Oncorhynchus mykiss)
Antibody Dilution WB (1:500), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein A Purified
Storage Buffer PBS, 0.02% sodium azide
Concentration 1 mg/ml
Specificity Detects the N-terminal domain of PUMA ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
Storage 4ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis 2 µg/ml of SPC-165 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion
Tissue Specificity N/A
Accession Number NP_001120712.1
Gene ID 27113
Swiss-Prot Q9BXH1
Field of Use For in vitro research use only.
