Product Description
Rabbit Anti-Human RAF1 (pSer296) Polyclonal
Size
Size 100 µl
Target RAF1 (pSer296)
Conjugate PerCP
Research Area Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | Cancer | Apoptosis | Cancer | Tumor Biomarkers | Oncoproteins | Growth Factor Receptors | Cell Signaling | Metabolism | Mitochondrial Metabolism | Mitochondrial Markers
Alternative Name c Raf Antibody, C-raf Antibody, C-Raf proto-oncogene, serine/threonine kinase Antibody, CMD1NN Antibody, Craf 1 transforming gene Antibody, cRaf Antibody, Craf1 transforming gene Antibody, EC 2.7.11.1 Antibody, kinase Raf1 Antibody, Murine sarcoma 36
Category Antibodies
Product Type Polyclonal Antibody
Immunogen A phospho-specific peptide corresponding to residues surrounding Ser296 of human RAF1 (AA293-299)
Immunogen Species Human
Applications WB, AM
Host Rabbit
Isotype N/A
Species Reactivity Human
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Concentration N/A
Specificity Detects ~73kDa.
Storage -20ºC
Shipping Blue Ice or 4ºC
Certificate of Analysis A 1:250 dilution of SPC-1071 was sufficient for detection of RAF1 (pSer296) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm, Cell Membrane, Mitochondrion, Nucleus
Tissue Specificity In skeletal muscle, isoform 1 is more abundant than isoform 2
Accession Number NP_002871
Gene ID 5894
Swiss-Prot P04049
Field of Use For in vitro research use only.