Synthetic peptide of Human SMAD3 (110-210aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Immunogen Species:
Human
Applications:
WB, IHC
Isotype:
NA
Species Reactivity:
Human
Antibody Dilution:
WB (1:1000); IHC (1:50); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration:
1 mg/ml
Specificity:
Detects 48 kDa, additional bands due to post translational modifications.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:1000 dilution of SPC-729 was sufficient for detection of SMAD3 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
SpecificityDetects 48 kDa, additional bands due to post translational modifications.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:1000 dilution of SPC-729 was sufficient for detection of SMAD3 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.