Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group.
Immunogen Species:
Mouse
Applications:
WB, ICC/IF, IP
Host:
Rat
Isotype:
IgG2c
Species Reactivity:
Mouse, Rat, Sheep, Rabbit, Hamster, Dog, Bovine
Antibody Dilution:
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~60kDa. Also detects ~92kDa. The addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences. This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. I
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml of SMC-478 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular Localization:
Cytoplasm
Accession Number:
NP_038714.2
Gene ID:
21454
Swiss-Prot:
P11983
Field of Use:
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
ImmunogenPurified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group.
Immunogen SpeciesMouse
ApplicationsWB, ICC/IF, IP
HostRat
IsotypeIgG2c
Species ReactivityMouse, Rat, Sheep, Rabbit, Hamster, Dog, Bovine
Antibody DilutionWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
SpecificityDetects ~60kDa. Also detects ~92kDa. The addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences. This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. I
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis1 µg/ml of SMC-478 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular LocalizationCytoplasm
Accession NumberNP_038714.2
Gene ID21454
Swiss-ProtP11983
Field of UseNot for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.