WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification:
Protein G Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular Localization:
Cytoplasm
Accession Number:
NP_038714.2
Gene ID:
21454
Swiss-Prot:
P11983
Field of Use:
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
SpecificityDetects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular LocalizationCytoplasm
Accession NumberNP_038714.2
Gene ID21454
Swiss-ProtP11983
Field of UseNot for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.