Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
Immunogen Species:
Human
Applications:
WB, IHC, ICC/IF, FCM, FACS
Host:
Rabbit
Isotype:
N/A
Species Reactivity:
Human, Mouse
Antibody Dilution:
WB (1:500), IHC (1:50); optimal dilutions for assays should be determined by the user.
Purification:
Protein A Purified
Storage Buffer:
PBS, 50% glycerol, 0.09% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
SpecificityDetects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of Analysis2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.