Product Description
The vertebrate nuclear pore complex (NPC) is a macromolecular assembly of protein subcomplexes forming a structure of eightfold radial symmetry. The NPC core consists of globular subunits sandwiched between two coaxial ring-like structures of which the ring facing the nuclear interior is capped by a fibrous structure called the nuclear basket. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. TPR localizes to intranuclear filaments of the NPC, and is a component of the cytoplasmic fibrils of the NPC implicated in nuclear protein import. Experimental data suggest that TPR is tethered to intranuclear filaments of the NPC by its coiled coil domain leaving the acidic COOH terminus free to interact with soluble transport factors and mediate export of macromolecules from the nucleus.
Biovision | 3781 | TPR Antibody DataSheet
Antibody Target: TPR
Target Alternative Name: TPR, Translocated Promoter Region
Tag Line:
Category: TPr
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human
Immunogen Sequence: Synthetic peptide of human TPR
Accession #: NP_003283.1
Gene ID: 7175
Appearance: Colorless liquid
Form: Liquid
Concentration: N/A
Formulation: 100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
Purification: Affinity purified
Application: Western blot
Positive Control:
Application And Usages: Western blotting (1:500 1:2000). However, the optimal concentrations should be determined individually. HeLa and 293T cell lysates can be used as positive controls. The antibody recognizes the TPR of human origin. Reactivity to other species has not been tested.
Country of Animal Origin: China
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Verified
Immunocytochemistry Verified: Not Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified: Not Verified
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified:
Flow Cytometry Verified: Not Verified