WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification:
Peptide Affinity Purified
Storage Buffer:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration:
1 mg/ml
Specificity:
Detects ~10kDa. It also recognizes ubiquinated proteins.
Storage:
-20ºC
Shipping:
Blue Ice or 4ºC
Certificate of Analysis:
A 1:1000 dilution of SPC-119 was sufficient for detection of free ubiquitin in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
SpecificityDetects ~10kDa. It also recognizes ubiquinated proteins.
Storage-20ºC
ShippingBlue Ice or 4ºC
Certificate of AnalysisA 1:1000 dilution of SPC-119 was sufficient for detection of free ubiquitin in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.