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Product Description
EnzyChrom™ Phospholipd Assay Kit
Application: For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.
Key Features:
Sensitive. Use 20 μL samples. Linear detection range: colorimetric assay 3 - 200 μM, fluorimetric assay 0.6 - 20 μM phospholipid.
Method: OD570nm, or FL530/585nm
Samples: Biological
Species: All
Size: 100 tests
Detection Limit: OD, FL: 3, 0.6 μM
Shelf Life: 6 months
More Details:
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples.
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.
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