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Product Description
EnzyFluo™ NADP/NADPH Assay Kit
Application: For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.
Key Features:
- Sensitive and accurate. Detection limit of 0.01 μM and linearity up to 1 μM NADP+/NADPH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method: FL530/585nm
Samples: Cell, tissue extracts etc
Species: All
Size: 100 tests
Detection Limit: 0.01 μM
Shelf Life: 6 months
More Details:
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable.
BioAssay Systems EnzyFluo™ NADP+/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and with minimal interference (<1%) by NAD+/NADH. Our assay is a convenient method to measure NADP+, NADPH and their ratio.
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