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Product Description
FitAmp Paraffin Tissue Section DNA Isolation Kit DataSheet
The FitAmp™ Paraffin Tissue Section DNA Isolation Kit is a complete set of essential components that enables researchers to efficiently isolate DNA from formalin-fixed and paraffin-embedded tissue section samples. It is suitable for isolating tiny amounts of DNA from microdissection samples, fresh tissue sections, formalin-fixed and paraffin-embedded tissues, plasma, serum, body fluids, etc. Depending on sample types, the entire procedure can be completed within 2 hours.
- Fast - Entire procedure can be finished in just 2 hours with consistent isolation conditions.
- Effective -High efficiency of DNA isolation from tissue sections containing tiny amounts of DNA (as low as 1 ng).
- Easy - No requirement for pre-deparaffinization.
- Reliable – Our specially designed F-Spin Columns allow for DNA to be conveniently recovered.
- Safe -Use of non-toxic reagents and no phenol chloroform.
This kit can also be employed as the initial step in our popular DNA methylation & hydroxymethylation quantitation kits: MethylFlash Global DNA Methylation 5-mC ELISA Easy Kit, and the MethylFlash Global DNA Methylation 5-hmC ELISA Easy Kit.
Background
Rapid and efficient preparation of DNA is crucial to a successful, DNA-based experiment. DNA samples can be prepared from cells, tissues, and body fluids, and are the most commonly used starting material for the majority of DNA methylation applications and assays. The FitAmp™ Paraffin Tissue Section DNA Isolation Kit ensures ready-to-use DNA no matter what the downstream application is.
Principle & Procedure
This kit simply applies our proprietary DNA isolation buffer to a selected microscopic tissue area. The area is removed and transferred into a tube. After treatment with DNA digestion buffer, the DNA is easily recovered in 8-20 µl by our specially designed F-Spin Column. DNA is then ready for down-stream application.
Starting Materials
Input amount of tissue can be as low as 1ng with the optimal range between 10 ng and 1 µg. This kit can easily yield 1 ng to 4 µg of DNA in spin column format, which can be used for any molecular biology procedures such as PCR, restriction digestion, cloning and sequencing, etc
